Conventional vaccines work by introducing weakened or "killed" virions into the body so the immune system can recognize and form antibodies against their exposed structural proteins. You can think of the proteins on the surface of a virus as keys to a specific type of lock, which are the receptor proteins found on the surfaces of human cells. The type of cell most commonly infected by a virus is decided by the level of genetic expression of that specific protein in that specific cell line, so some viruses favor different types of tissue over others. SARS-CoV-2 binds to ACE2 with its Spike protein, and since ACE2 is expressed by a very wide range of cell types (it's part of the osmoregulation system), it has tropism for a wide variety of different tissues. However, it mostly infects the vascular endothelium, the inner lining of blood vessels. In some cases, this causes sepsis, along with everything else that comes with sepsis (coagulopathy, fluid leaking into the lungs, etc.). For every virus, it's a little different. Ebola viruses bind to myeloid cells and the like through C-type lectins and TIM-1. You can actually (sort of) predict what cell lines a virus will preferentially infect by looking at the rate of expression of those receptors relative to other cells.
All viruses try and do the same thing when they enter the body. Bind to cells, get pulled inside, and basically order the cell at gunpoint to make more viruses instead of its own proteins. They're not actually "alive" because they don't have any replication machinery of their own. They're just free-floating instructions to make more of themselves. Kind of like life on pause.
Antibodies neutralize viruses by binding to their proteins, rendering them incapable of binding to anything else. If the surface proteins of a virus are like keys that go in specific locks, then antibodies are like a swarm of flying padlocks that snap over them. The other end of the antibody binds to a receptor on, for instance, a macrophage, which recognizes it as a neutralized pathogen and engulfs it. Macrophages and neutrophils are hilariously violent and annihilate their prey (bacteria and viruses and the like) by smothering them and dissolving them in what is basically a soup of peroxide and bleach. The proteins and membranes of the virus or bacteria or fungus or whatever are attacked by reactive oxygen species stripping electrons out of them, which fragments their proteins and bursts their membranes.
Conventional vaccines work by introducing pre-weakened virus with limited or no replication ability into the body, which forms an antibody response without an actual infection.
Messenger RNA vaccines do not work that way. They don't consist of any part of a virus. They're liposomes containing messenger RNA. The way they're supposed to work, they're injected into the deltoid muscle of someone's shoulder, transfect deltoid muscle cells with the mRNA, and then, those cells' ribosomes read the mRNA, link together amino acids in the specified codon sequence, and express SARS-CoV-2 Spike proteins. Then, those Spike proteins travel to the surface of the cell and become membrane-bound proteins, or they're packaged up and exported in vesicles. Eventually, the body recognizes them as foreign proteins and makes antibodies against them.
This whole concept is basically like using human cells as bioreactors. You could do this same exact process by transfecting, for instance, E. coli bacteria or yeast in a vat, and then collecting the produced Spike proteins and purifying them and injecting them into someone. That would be a protein subunit vaccine, like Novavax. The Pfizer and Moderna vaccines use you as the vat of E. coli or yeast. Your own cells become the bioreactors. As a matter of fact, this is the exact language that DARPA uses to describe the process.
Technically, this process can be used to force cells to produce any arbitrary protein in a one-shot process. DARPA's ADEPT: PROTECT project involved using mRNA vaccines to make monoclonal antibodies instead of viral antigens, for instance, which would theoretically function just like mAb therapy. Because it's RNA, it's not supposed to integrate into the genome, so the translation of protein only occurs for as long as the mRNA exists, unlike, for instance, if you integrated new DNA into the chromatin in the nucleus of a cell in an active region and it started producing its own RNA.
Messenger RNA is also how cells produce their own proteins. The synthesis of proteins in cells basically works like this: a section of the chromosome is reeled out and exposed, RNA polymerase copies the DNA into an mRNA strand (transcription). The DNA is the storage form of genes, and the RNA is the working copy. The mRNA leaves the cell nucleus through pores that act as little gateways, and then ribosome subunits snap around it and start reading it, like a punch card or a reel of tape. The RNA contains three-letter codon groups which are tested by tRNAs. If there's no match, the tRNA bounces off. If there is a match, it latches on, deposits its amino acid onto the growing polypeptide chain, and then is ejected. Eventually, it hits the stop codon at the end, and the polypeptide detaches and folds in on itself to become a protein.
DNA has a double helix consisting of paired strands of adenine, guanine, cytosine, and thymine. RNA has a single strand of adenine, guanine, cytosine, and uracil. These are arranged into three-letter units called codons. There are 64 codons and 20 to 22 different amino acids used by different organisms. Since there are more codons than amino acids, some codons code for the same amino acid, of course.
Essentially, the geometry of a protein is decided by the intramolecular forces and bonds between different types of amino acids (and the intermolecular forces in the surrounding solvent, which is largely water). As you can imagine, there are theoretically an absolutely redonkulous number of possible combinations and protein geometries possible. The possible number of configurations of a protein 300 amino acids long, for instance, is much larger than the number of atoms in the universe.
The mRNA vaccines are like steps two and three of this process, only rather than the mRNA coming from the nucleus as a product of the individual's genes, it's coming from a little bubble of oil full of mRNA fusing with the cell from the outside and depositing its cargo into the cytoplasm that way. It's exogenous mRNA.
There are many, many problems with this approach.
For one thing, the cationic, PEGylated lipid nanoparticles in these vaccines are not normal fats like what you typically find in the body. They are synthetic oils, like SM-102, conjugated with polyethylene glycol, and are extremely inflammatory, to the point where they're considered "self-adjuvants" that don't need metal adjuvants like aluminum to stimulate an inflammatory response. Sometimes, these LNPs can cause anaphylaxis just like someone with peanut allergies chowing down on a peanut butter sandwich, so basically, anyone who takes one of these vaccines should have someone standing by with an Epi-pen in case they go down and their airway starts closing off.
Normally, the body is very hostile to exogenous mRNA, so they use mRNA where all of the Uracil (U) is replaced with Pseudouridylyl (Ψ). This theoretically avoids the toll-like receptor response. Normally, TLRs act like little smoke alarms on the surfaces of cells that sense dangerous biomolecules, and foreign RNA is one of the things that sets them off, causing that cell to start spewing cytokines. Inflammatory cytokines are how a cell tells the immune system "Help me, oh my god, I'm surrounded by viruses/bacteria/fungi/protists/cancer cells!" The process of the activation of inflammatory transcription factors is like a homeowner frantically dialing 911 after a burglar has thrown a brick through the window. If you're trying to dose someone with therapeutic mRNA, you don't want this to happen. You want the mRNA to evade TLRs. This mRNA vaccine business is largely based on a 2005 paper by one Katalin Kariko, who observed that TLRs were not activated by the presence of pseudouridylated mRNA, where the Uracil had been replaced with the
In my investigations, I surmised that pseudouridylated and synthetically capped mRNA may actually behave like a TLR blocker, rather than merely evading TLRs. Now, the cell doesn't realize that anything is wrong at all, and has lost this valuable surveillance tool (at least until it gets rid of the damaged TLRs and expresses new ones). This means impaired viral and tumor surveillance.
www.news-medical.net
In the first half of last year, Stephanie Seneff and Peter McCullough came out with this paper:
www.ncbi.nlm.nih.gov
MicroRNAs are crucial to fetal development and their dysregulation could cause severe neurological issues.
www.ncbi.nlm.nih.gov
SARS-CoV-2 Spike is a nasty toxin. It has a superantigenic region, which is hyper-inflammatory, it has amyloidogenic motifs that generate amyloid fibrils (and giant amyloid-fibrin clots) when broken up by enzymes like trypsin and neutrophil elastase, it's prionogenic, and it even binds to bacterial lipopolysaccharides. Not to mention, the lipid nanoparticles travel all over the body and transfect all sorts of cell lines. Brain cells, heart cells, bone marrow, you name it. Any cells that express this protein on their surfaces will be destroyed by the immune system. No exceptions.
However, the truth is, we don't even know the purity of the mRNA in these vaccine formulations or whether or not they even translate Spike proteins at all, or what final conformation those proteins adopt after the sequence is codon-optimized.
sashalatypova.substack.com
sashalatypova.substack.com
sashalatypova.substack.com
sashalatypova.substack.com
It may be that some of the mRNA strands translate into Spike, and some translate into other junk proteins with activity we can't even characterize.
The thing is, the cells expressing these proteins on their surfaces are human. The way the immune system normally works, the expectation is that if you see a particle expressing foreign proteins, that's a virus or bacterium. The immune system doesn't recognize proteins. It recognizes the whole object, like this:
So the reason why you have this class switch from IgG3 to IgG4 and immune tolerance is likely because the immune system is figuring out "wait a minute, I'm attacking my own cells, I need to tolerate this protein because it's not a part of a virus, it's a part of my own cells". Needless to say, that's not something you want to happen with a viral protein.
This is not a vaccination campaign. It is deliberate population culling and democide. Murder by government.
All viruses try and do the same thing when they enter the body. Bind to cells, get pulled inside, and basically order the cell at gunpoint to make more viruses instead of its own proteins. They're not actually "alive" because they don't have any replication machinery of their own. They're just free-floating instructions to make more of themselves. Kind of like life on pause.
Antibodies neutralize viruses by binding to their proteins, rendering them incapable of binding to anything else. If the surface proteins of a virus are like keys that go in specific locks, then antibodies are like a swarm of flying padlocks that snap over them. The other end of the antibody binds to a receptor on, for instance, a macrophage, which recognizes it as a neutralized pathogen and engulfs it. Macrophages and neutrophils are hilariously violent and annihilate their prey (bacteria and viruses and the like) by smothering them and dissolving them in what is basically a soup of peroxide and bleach. The proteins and membranes of the virus or bacteria or fungus or whatever are attacked by reactive oxygen species stripping electrons out of them, which fragments their proteins and bursts their membranes.
Conventional vaccines work by introducing pre-weakened virus with limited or no replication ability into the body, which forms an antibody response without an actual infection.
Messenger RNA vaccines do not work that way. They don't consist of any part of a virus. They're liposomes containing messenger RNA. The way they're supposed to work, they're injected into the deltoid muscle of someone's shoulder, transfect deltoid muscle cells with the mRNA, and then, those cells' ribosomes read the mRNA, link together amino acids in the specified codon sequence, and express SARS-CoV-2 Spike proteins. Then, those Spike proteins travel to the surface of the cell and become membrane-bound proteins, or they're packaged up and exported in vesicles. Eventually, the body recognizes them as foreign proteins and makes antibodies against them.
This whole concept is basically like using human cells as bioreactors. You could do this same exact process by transfecting, for instance, E. coli bacteria or yeast in a vat, and then collecting the produced Spike proteins and purifying them and injecting them into someone. That would be a protein subunit vaccine, like Novavax. The Pfizer and Moderna vaccines use you as the vat of E. coli or yeast. Your own cells become the bioreactors. As a matter of fact, this is the exact language that DARPA uses to describe the process.
Technically, this process can be used to force cells to produce any arbitrary protein in a one-shot process. DARPA's ADEPT: PROTECT project involved using mRNA vaccines to make monoclonal antibodies instead of viral antigens, for instance, which would theoretically function just like mAb therapy. Because it's RNA, it's not supposed to integrate into the genome, so the translation of protein only occurs for as long as the mRNA exists, unlike, for instance, if you integrated new DNA into the chromatin in the nucleus of a cell in an active region and it started producing its own RNA.
Messenger RNA is also how cells produce their own proteins. The synthesis of proteins in cells basically works like this: a section of the chromosome is reeled out and exposed, RNA polymerase copies the DNA into an mRNA strand (transcription). The DNA is the storage form of genes, and the RNA is the working copy. The mRNA leaves the cell nucleus through pores that act as little gateways, and then ribosome subunits snap around it and start reading it, like a punch card or a reel of tape. The RNA contains three-letter codon groups which are tested by tRNAs. If there's no match, the tRNA bounces off. If there is a match, it latches on, deposits its amino acid onto the growing polypeptide chain, and then is ejected. Eventually, it hits the stop codon at the end, and the polypeptide detaches and folds in on itself to become a protein.
DNA has a double helix consisting of paired strands of adenine, guanine, cytosine, and thymine. RNA has a single strand of adenine, guanine, cytosine, and uracil. These are arranged into three-letter units called codons. There are 64 codons and 20 to 22 different amino acids used by different organisms. Since there are more codons than amino acids, some codons code for the same amino acid, of course.
Essentially, the geometry of a protein is decided by the intramolecular forces and bonds between different types of amino acids (and the intermolecular forces in the surrounding solvent, which is largely water). As you can imagine, there are theoretically an absolutely redonkulous number of possible combinations and protein geometries possible. The possible number of configurations of a protein 300 amino acids long, for instance, is much larger than the number of atoms in the universe.
The mRNA vaccines are like steps two and three of this process, only rather than the mRNA coming from the nucleus as a product of the individual's genes, it's coming from a little bubble of oil full of mRNA fusing with the cell from the outside and depositing its cargo into the cytoplasm that way. It's exogenous mRNA.
There are many, many problems with this approach.
For one thing, the cationic, PEGylated lipid nanoparticles in these vaccines are not normal fats like what you typically find in the body. They are synthetic oils, like SM-102, conjugated with polyethylene glycol, and are extremely inflammatory, to the point where they're considered "self-adjuvants" that don't need metal adjuvants like aluminum to stimulate an inflammatory response. Sometimes, these LNPs can cause anaphylaxis just like someone with peanut allergies chowing down on a peanut butter sandwich, so basically, anyone who takes one of these vaccines should have someone standing by with an Epi-pen in case they go down and their airway starts closing off.
Normally, the body is very hostile to exogenous mRNA, so they use mRNA where all of the Uracil (U) is replaced with Pseudouridylyl (Ψ). This theoretically avoids the toll-like receptor response. Normally, TLRs act like little smoke alarms on the surfaces of cells that sense dangerous biomolecules, and foreign RNA is one of the things that sets them off, causing that cell to start spewing cytokines. Inflammatory cytokines are how a cell tells the immune system "Help me, oh my god, I'm surrounded by viruses/bacteria/fungi/protists/cancer cells!" The process of the activation of inflammatory transcription factors is like a homeowner frantically dialing 911 after a burglar has thrown a brick through the window. If you're trying to dose someone with therapeutic mRNA, you don't want this to happen. You want the mRNA to evade TLRs. This mRNA vaccine business is largely based on a 2005 paper by one Katalin Kariko, who observed that TLRs were not activated by the presence of pseudouridylated mRNA, where the Uracil had been replaced with the
Suppression of RNA Recognition by Toll-like Receptors: The Impact of Nucleoside Modification and the Evolutionary Origin of RNA
DNA and RNA stimulate the mammalian innate immune system through activation of Toll-like receptors (TLRs). DNA containing methylated CpG motifs, however, is not stimulatory. Selected nucleosides in naturally occurring RNA are also methylated or otherwise modified, but the immunomodulatory...
www.cell.com
In my investigations, I surmised that pseudouridylated and synthetically capped mRNA may actually behave like a TLR blocker, rather than merely evading TLRs. Now, the cell doesn't realize that anything is wrong at all, and has lost this valuable surveillance tool (at least until it gets rid of the damaged TLRs and expresses new ones). This means impaired viral and tumor surveillance.
Research suggests Pfizer-BioNTech COVID-19 vaccine reprograms innate immune responses
Researchers in The Netherlands have warned that Pfizer-BioNTech’s coronavirus disease 2019 (COVID-19) vaccine induces complex reprogramming of innate immune responses that should be considered in the development and use of mRNA-based vaccines.
www.news-medical.net
In the first half of last year, Stephanie Seneff and Peter McCullough came out with this paper:
Innate immune suppression by SARS-CoV-2 mRNA vaccinations: The role of G-quadruplexes, exosomes, and MicroRNAs
The mRNA SARS-CoV-2 vaccines were brought to market in response to the public health crises of Covid-19. The utilization of mRNA vaccines in the context of infectious disease has no precedent. The many alterations in the vaccine mRNA hide the mRNA from ...
www.ncbi.nlm.nih.gov
MicroRNAs are crucial to fetal development and their dysregulation could cause severe neurological issues.
MicroRNAs and neuronal development
The importance of the involvement of non-protein coding RNAs in biological processes has become evident in recent years along with the identification of the transcriptional regulatory mechanisms that allow them to exert their roles. MicroRNAs (miRNAs) ...
www.ncbi.nlm.nih.gov
SARS-CoV-2 Spike is a nasty toxin. It has a superantigenic region, which is hyper-inflammatory, it has amyloidogenic motifs that generate amyloid fibrils (and giant amyloid-fibrin clots) when broken up by enzymes like trypsin and neutrophil elastase, it's prionogenic, and it even binds to bacterial lipopolysaccharides. Not to mention, the lipid nanoparticles travel all over the body and transfect all sorts of cell lines. Brain cells, heart cells, bone marrow, you name it. Any cells that express this protein on their surfaces will be destroyed by the immune system. No exceptions.
However, the truth is, we don't even know the purity of the mRNA in these vaccine formulations or whether or not they even translate Spike proteins at all, or what final conformation those proteins adopt after the sequence is codon-optimized.
Did Pfizer Perform Safety Testing for its Covid-19 mRNA Vaccine in Preclinical Studies?
Evidence of Scientific and Regulatory Fraud
sashalatypova.substack.com
Nobody Knows What is in the Vials
Covid-19 injections are dangerous, non-compliant biological materials. Their production must be stopped until a full investigation can be done.
sashalatypova.substack.com
Intent to Harm
“mRNA Vaccine Approval” was a farce. Deaths and injuries are real and intentional.
sashalatypova.substack.com
mRNA Injections as a Dual-Use Technology – Assessment of Threat of Misuse as Biological and Chemical Weapons.
Can adulterated and/or weaponized bio-chemical substances be definitively excluded from mRNA injections currently marketed as “Covid 19-Vaccines”?
sashalatypova.substack.com
It may be that some of the mRNA strands translate into Spike, and some translate into other junk proteins with activity we can't even characterize.
The thing is, the cells expressing these proteins on their surfaces are human. The way the immune system normally works, the expectation is that if you see a particle expressing foreign proteins, that's a virus or bacterium. The immune system doesn't recognize proteins. It recognizes the whole object, like this:
So the reason why you have this class switch from IgG3 to IgG4 and immune tolerance is likely because the immune system is figuring out "wait a minute, I'm attacking my own cells, I need to tolerate this protein because it's not a part of a virus, it's a part of my own cells". Needless to say, that's not something you want to happen with a viral protein.
This is not a vaccination campaign. It is deliberate population culling and democide. Murder by government.
